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Collection and preparation of cell plasma membranes and assessment of G protein conformational change was performed using a previously established method 27, 35

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Many contain hydrophobic regions that readily bind to plastic surfaces, pulling active peptide out of solution and depositing it on the container walls

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Concurrently, pro-inflammatory cytokines regulate the activity of key enzymes in tryptophan metabolismindoleamine IDO and TDOpromoting the production of kynurenine pathway metabolites such as KYNA