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Moreover, we observed the absence of catalase activity in the parasite

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In brief, serum samples (2.5 L) were incubated with 250 L of the working solution containing 4-aminoantipyrine, glucose oxidase, and sodium 3,5-dichloro-2-hydroxybenzenesulfonate at 37C for 10 min protected from light, followed by the detection of the absorbance at 505 nm with a microplate reader (EMax Plus, Molecular Devices, Sunnyvale, CA, USA)

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& van Raalte, D.H