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In a very recent paper, Akiyama et al

Purified GR was used in GR activity measurement using the method reported by Greer with minor modifications (Greer and Perham, 2.6

Moreover, natural plant extracts such as HT have been shown to exert significant anti-tumour activity via the ferroptosis pathway

Immunofluorescence staining Fibroblasts were fixed, permeabilised, blocked, and incubated overnight at 4 C with primary antibodies targeting p53-pS15 and FOXO4 shown in Table 2, and developed using Alexa Fluor 488-conjugated donkey anti-mouse immunoglobulin G (1:100