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We used 8 week old female C57BL/6 humanized microbiota mice established by Collins et al

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Malnutrition in cirrhotic patients is characterized by the loss of skeletal muscle and lean body mass (sarcopenia), adipose (fat) tissue (adipopenia), or both (hepatic cachexia), and does not respond to adequate dietary intake of fats or protein

The modest reduction in triagonist-induced insulin secretion observed in Gcgr / mouse islets was mirrored by comparable reductions in insulin secretion observed with other secretagogues