Mechanisms controlling cellular and systemic iron homeostasis
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Methods Antibodies Anti-MBP tag mouse monoclonal antibody and anti c-Jun rabbit monoclonal antibody was used at 1:1000 dilution (Beyotime, AF2912, AF1612), anti-ubiquitin was used at 1:20000 dilution (Abcam, ab134953), anti-GST tag mouse monoclonal antibody was used at 1:5000 dilution (Yeason, 30901ES50), HRP-conjuagated FLAG-tag mouse monoclonal antibody was used at 1:1000 dilution (Yeason, 30502ES60)
Storage & Handling Instructions: Lyophilized (Dry) Storage: Store at -20C in a dry, desiccated environment
It interacts with enzymes such as neutral endopeptidase and aminopeptidase N, which influence peptide metabolism in tissues
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