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Assays were performed in triplicate for each sample on a Roche Lightcycler 480 system (Roche, Basel, Switzerland) using Realtime Ready DNA probes Mastermix supplemented with ResoLight Dye (Roche) according to the manufacturers protocol

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Berberine-induced LDLR up-regulation involves JNK pathway

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Melanin is the pigment that gives our skin, hair, and eyes their color

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