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For reducing SDS-PAGE, 2 g of protein was mixed with 3 L of 4 Bolt LDS Sample Buffer (Invitrogen), 1.2 L of 10 Bolt Sample Reducing Agent (Invitrogen), and Milli-Q water to a total volume of 12 L, and the samples were incubated for 10 min at 95 C

You may feel less hungry, eat smaller meals, or snack less often

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GPX activity was expressed in M TNB ( = 13.6 mm 1 cm 1 ) min 1 mg 1 protein

Therefore, despite the fact that decreasing the dosing frequency from q1wk to q2wk decreases the total amount of drug taken over time by one half, the model predicts that patients retain 72% of their weight loss compared with q1wk