E., Lee, H., Toribio, M., Stanley, T
The supernatant was incubated with Rho 1D4 affinity resin overnight at 4 C
We have recently demonstrated that a high dietary fat intake increased prostate 5 -reductase-2 gene expression and circulating DHT levels in the rat without significant alteration in prostate and hepatic 5 -reductase-1 gene expression and plasma testosterone concentration 94 , suggesting that alteration in prostate 5 -reductase activity after a high-fat diet may be a potential mechanism for dietary fat stimulation of prostate growth and pathogenesis (Fig
One of the major drawbacks of this method is the variability of the LDL samples, which can vary between donors because LDL is isolated from blood samples
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