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RNA extraction and qRTPCR analysis Cells were grown to 80% confluency in 75 cm 2 flasks, harvested by trypsinisation, counted using a haemocytometer, and 1 10 7 cells used for RNA extraction using a Qiagen RNeasy mini kit (Qiagen), following the manufacturers protocol for mammalian cells, with additional on column DNAse digestion (RNAse free DNAse kit, Qiagen)

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(no photo available) 96 tests $974 KT-50596 Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5 ELISA Kit (NOX5), Human For quantitative detection in human samples

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