In MCF-7 breast cancer cells, gold (Au) NPs smaller than 10 nm (2 and 6 nm) had the deeper internalization into the cell nucleus, while larger NPs (10 and 16 nm) were only accumulated in the cytoplasm (Huo et al., 2014)
Reconstitute with sterile bacteriostatic water or appropriate laboratory buffer Add solvent slowly along the vial wall to minimize foaming Gently swirl until the peptide is dissolved Store reconstituted solutions at 3646 F (28 C) Prepare aliquots where appropriate to reduce freezethaw cycles Careful preparation procedures help maintain peptide stability and solution consistency during laboratory experiments
J Phys Org Chem 17:529536 Orlowski M, Meister A (1963) Gamma-glutamyl- p -nitroanilide: a new convenient substrate for determination and study of l- and d-gamma-glutamyltranspeptidase activities
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If, on the other hand, the plant cell has a high level of solvent and the material surrounding the cell has a high concentration of solute, we describe that cell as being in a hypertonic solution and the plant cell will lose water, resulting in flaccid plant tissue
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