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When pregnant rats were kept on a copper-deficient diet, the embryos displayed low SOD activity, increased super oxide anion radical level, and higher incidence of DNA damage and malformations [34]

Aliquots of protein lysates were electrophoresed on a sodium dodecyl sulfatepolyacrylamide gel, transferred from the gel onto a PVDF membrane, and blocked with 5% BSA for 1 h