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Cytotoxicity profiling in vitro shows a dose-dependent pattern: cell viability holds within research-relevant concentration ranges but declines meaningfully above approximately 1 mM in culture, a threshold well outside the concentration windows described as biologically productive in the wound healing and gene expression literature

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Glutathione protects cells from oxidative damage while NAD+ provides the energy for cellular function

The Broad Institute Connectivity Map analyzed 1,309 bioactive molecules and selected GHK-Cu as the single best agent for reversing metastatic colon cancer gene signatures

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Proc Natl Acad Sci USA (1981) 78:22304