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Immunoblot analysis For detection of Gclc (Santa Cruz Biotechnology sc-390811), Pdha-1 (Abcam ab168379), DRP1 (Abcam ab184247), eIF4E (Abcam ab33766), p-eIF4E (Abcam ab76256), 4E-BP1 (Cell signaling 9452), p-4E-BP1 (Cell signaling 2855), DRP1 (Abcam ab184247), mTOR (Cell Signaling 2972), p-mTOR (Ser2448) (Cell Signaling 2971), p70 S6 (Cell Signaling 9202), p-p70 S6 (Thr421/Ser424) (Cell Signaling 9204), 15 10 6 cells were lysed with lysis buffer (CST 9803S) and protein/phosphatases inhibitors as per manufacturers protocol and blotted as described 44

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After additional PBS washes, non-specific binding sites were blocked by incubation with BSA for 30 min

doi: 10.1212/WNL.0000000000008785 44 JonklaasJ

Apart from their noteworthy role in the ecosystems recyclers and decomposers, the mushrooms have various biological activities including antibacterial, anticancer, insecticidal, antioxidant, anti-proliferative, anticoagulant, antifungal, antidiabetic, hepatoprotective, antiparasitic, antiviral, and anti-inflammatory properties (Atomachi, 1988)