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Protein extraction and characterization A detailed description of our protein-related research methods, including extraction, proteomics analysis, liquid chromatography-mass spectrometry (LC-MS) analysis, database searching, criteria for identification, and quantitative proteomics, is provided in Supplementary Material

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Oxidant and antioxidant status in coronary artery disease

For reference, these are the Recommended Dietary Allowances (RDA) for vitamin B12 by sex and age group indicating the daily amount needed for healthy individuals ( Men 1418: 2.4 mcg Men 19+: 2.4 mcg Women 1418: 2.4 mcg Women 19+: 2.4 mcg During pregnancy: 2.4 mcg During lactation: 2.8 mcg SUMMARY The RDA for vitamin B12 for most people is 2.4 mcg per day

Methodology: We developed and validated an HPLC-UV method for precision, accuracy, linearity, limits of detection (LOQ), and limits of quantitation (LOQ) for the quantitative analysis of both GSH and four associated impurities A-D