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Islets were handpicked by a micropipette under the microscope and cultured in RPMI 1640 medium (11875093, Life Technologies) overnight

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nucleus basalis of Meynert, central gray (especially medial), amygdala, spinal cord, the bed nuclei of the stria terminalis, the shell of the nucleus accumbens and the dorsal raphe nuclei

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These receptors become stimulated from many substances, including heroin, prescription pills and other opioids

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