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The absorbance at 450 nm was collected using a BioTek Cytation 5 imaging plate reader running Gen5 3.13 software

DR7 is also associated with the increase of colon short chain fatty acids (SCFA) which reverses intestinal flora disorder (HFD) by increasing the abondance of Bacteroides and Bifidobacteriales and reduces the abundance of Firmicutes and Closteridiales and thus regulates lipid metabolism

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doi: 10.1073/pnas.0502979102

10.5402/2012/376023 347 ObesoJ

Minireview: Endocannabinoids and gonadal hormones: bidirectional interactions in physiology and behavior