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After OD at 600 nm reaches to 0.7, 0.5 mM IPTG was added into the Luria-Bertani Broth medium for 5 h at 37 C

These peptides have also been shown to exert anti-inflammatory properties, probably due to the prevention of bacterial translocation in the gut by the increase of TJ expression, the suppression of pro-inflammatory cytokines released by T cells, monocytes, and dendritic cells, and the prevention of macrophage activation and migration [28]

Further, the sensitive coordinate bond of catechol-Fe and dynamic Schiff base bonds improved the mechanical properties of the hydrogel as well as their injectability and self-healing properties, thus making the adhesive hydrogel a smart wound sealant to heal infected wounds and improve tissue regeneration in vivo

Patients on liraglutide lost 5.7% BW at 32 weeks compared to 1.6% for those on placebo 20