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mSphere 5 (1), e00585-19

40 L- 4050

Majumder P, Duchen MR, Gale JE

DSIP is not unique in its ability to exhibit diurnal variations in antiepileptic activity

Benzyl Alcohol Concentration: The concentration of the preservative is a key factor

GSH Quantification Glutathione and GSSG were measured in lysates from cells in 6-well plates as previously described ( ROS Quantification by Spin-Trapping and EPR Spectroscopy Cells (5 10 6 /0.1 ml) were stimulated for 2 h with LPS, then incubated with 50 mM 5-tert-butoxycarbonyl-5-methyl-1-pyrroline-N-oxide (BMPO), synthetized as described previously ( Cell Viability Assay Cells were seeded in 96-well plates at 25,000/well in complete medium