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Autoantibodies have been shown to predate clinical disease by years: anti-CCP antibodies, highly specific for rheumatoid arthritis, can be detected up to a decade before diagnosis 1

Immunoblotting Tissue and cell lysates were extracted from cells using RIPA buffer with protease inhibitor (Thermo Fisher Scientific, MA, USA)

Research has documented reduced fat mass, decreased adipocyte size, and shifts in adipose tissue gene expression patterns toward fat-mobilizing rather than fat-storing phenotypes

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Once in position, the cannula was secured to the skull with two stainless-steel screws and dental cement

ADK, adenylate kinase