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For skin tissue, mRNA libraries were prepared using the Illumina Stranded mRNA Prep Ligation Kit (Cat #20040534, Illumina, San Diego, CA, USA, and the resulting libraries were quantified with the Qubit dsDNA Assay Kit (Cat #Q35853, Qiagen, MD, USA)

Researchers use SGLT2-, SGLT1-, URAT1-, and GLUT9-knockout mouse models to investigate the UA-lowering effect of the SGLT2 inhibitor canagliflozin, and show that the mechanism of this effect involved intraluminal glucose transmission and inhibition of the proximal tubule urate transporter URAT1 (Novikov et al., 2019

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