DOI: 10.5599/admet.4.2.292 Substituting into the above aqueousintrinsic transformation equation, we get: The -based approach reproduces the classic HendersonHasselbalch expression but has the advantage of also scaling to complex cases with more than a single ionizable site
2.3 16S rRNA gene sequencing, read processing and annotation The V3-V4 region of the 16S rRNA gene was amplified with forward primer 5-TCGT CGGC AGCG TCAG ATGT GTAT AAGA GACA GCCT ACGG GNGG CWGC AG-3 and reverse primer 5-GTCT CGTG GGCT CGGA GATG TGTA TAAG AGAC AGGA CTAC HVGG GTAT CTAA TCC-3, with adapter sequences for compatibility with the Illumina Nextera XT Index kit
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