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According to the step a), the expression vector may already have desired restriction sites for ligation or can be introduced into expression vector by using restriction enzyme

By adopting ion-exchange separation and purification, the preparation method has the characteristics of being high in degree of separation, good in purification effects, few in impurities and easy to operate

Example 16: Cleavage and purification of Semaglutide [0082] 180 g of Semaglutide Wang resin obtained in Example 11 was taken and added into a 3 L reaction kettle

c) recovering the expressed Arg 34 GLP-l (9-37) peptide, d) optionally, purifying the expressed Arg 34 GLP-l (9-37) peptide

das 2-CTC-Harz einen Substitutionsgrad von 0,2 - 0,6 mmol/g aufweist

et la rsine 2-CTC est couple par un procd DiPEA