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Fluorescence images of unstained SDS gels were recorded on a ChemiDoc MP (Bio-Rad) and band intensity was analyzed using ImageLab 6.1 (Bio-Rad)

This is a nice upgrade from the previous version, which was 180 days, and the original, which was only 90 days

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These can range from mild discomfort to severe pain and limited mobility

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Three weeks later, mice were terminally anaesthetised with 100 mg/kg pentobarbital and transcardially perfused with PBS containing 4% PFA, brains were collected, post-fixed in 4% PFA at 4 C overnight and cryoprotected in 30% sucrose for 23 days