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The crude material was purified by Isolera flash column chromatography on silica via gradient elution, from 100% dichloromethane to 15% methanol in dichloromethane to produce CAM-S-CAM- d 15 TPP (9.1 mg, 27%) as a pale yellow fluffy/foamy solid

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There are many glutathione molecules conjugated to each agarose bead, so multiple GST-fusion proteins can bind to the same bead (Figure 3)

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