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Diet-induced-obese mice showed changes in body weight, fat mass, glucose handling, and liver pathology, while aged female mice showed improved grip strength

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By blocking NNMT, it shifts intracellular NAD+ and S-adenosylmethionine (SAM) levels, two cofactors tied to energy metabolism, redox balance, and gene regulation in laboratory models

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The compound showed an in vitro NNMT IC 50 of approximately 1.2 M and did not inhibit related methyltransferases at concentrations tested