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The same method has also been successfully used to analyze the stoichiometry of actinoporin EqtII complexes at the PM of living cells, showing that the protein does not organize into a unique oligomeric form but it exists as a mixture of oligomeric species including monomers, dimers, tetramers, and hexamers [10]

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Clean the injection site with alcohol, allow to dry, pinch the skin lightly if needed, insert the needle at a 45 to 90 degree angle depending on tissue depth, and inject slowly