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After centrifugation (15 min, 12,000 g, 4 C), the upper aqueous phase was carefully collected and transferred into a new tube containing 1 ml of freshly prepared 70% ethanol solution, mixed by pipetting, and loaded onto a RNeasy spin column (RNeasy mini kit, Qiagen)

Make one change at a time to identify what works

Think of it like this: a doctor needs an X-ray to confirm a broken bone before putting on a cast

Consult your healthcare provider for personalized advice

[1] [2] When neurological symptoms are present such as paraesthesia, numbness, balance problems, or cognitive changesmore aggressive treatment is required

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