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While ROS generation is a natural part of cellular metabolism, the balance between ROS production and antioxidant defenses is key to preventing oxidative stress and ensuring proper cellular function 54

2.5.4 Determination of NKCC1 The Rat NKCC1 ELISA kit (ELK 0482, Wuhan, China) quantitatively determined NKCC1 concentrations in brain tissue using a sandwich enzyme immunoassay

Cells were washed twice with fixative solution and then dropped onto chilled humid slides, where cells were left to dry overnight

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Inflammaging and neurodegenerative diseases: Role of NLRP3 inflammasome activation in brain atherosclerotic vascular disease

cardiovascular disease