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Thiol-reactive reagents such as N -ethylmaleimide, iodoacetic/iodoacetamide derivatives and nitric oxide donors can inactivate TST by modifying the catalytic cysteine via S -alkylation or S -nitrosylation, but they are not specific to this enzyme ( Azotobacter vinelandii rhodanese (RhdA) and inhibit its activity (Wrbel and Jurkowska, 2007)

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Exactly 100 l of blood serum was added to 200 l of 8.1% sodium dodecyl sulphate (SDS), followed by the addition of 500 l each of 0.8% thiobarbituric acid and acetic acid solution (2.5 M HCl, pH 3.4) into the test tube