Preclinical research demonstrated that NNMT inhibition with 5-Amino-1MQ reduced white adipose tissue mass by ~35% , shrank adipocyte size by 30-40%, and lowered plasma cholesterol by ~30% all without affecting food intake
Additionally, research published in Drug Testing and Analysis characterizing AOD9604 metabolism and confirming its C-terminal HGH fragment structure, supporting its classification as a non-IGF-1-stimulating lipolytic agent has been used by clinicians evaluating the compound for metabolic applications
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These results support a useful selectivity window for NNMT research, but they were obtained with a limited panel of related enzymes and do not rule out every possible off-target interaction
