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Both compounds belong to the incretin class, but Tirzepatide expands the mechanism beyond GLP-1 signalling

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After 48 h, DMEM was replaced with Neurobasal medium (Gibco, 21103049) containing 50 B27 (Gibco, 17504044), 100 glutamine (Invitrogen, 25030081), and 100 penicillin-streptomycin (Thermo, 15140122)