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After being cleaned of connective tissues, the heart was transferred into a Sylgard-coated prechilled (4 C) chamber containing Tyrodes solution and pinned down onto the Sylgard layer of the chamber
showed that nutritionally induced oxidative stress in mice fed an antioxidant-deficient diet enhances the pathogenesis of coxsackievirus B3 infection and the development of myocarditis 135
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