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The lysates were centrifuged (10.000 g , 30 min, 4 C) and the supernatants were loaded on equilibrated Ni-NTA agarose columns (Qiagen)
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Clin Cosmet Investig Dermatol 7:267274
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While incretin-based therapies seem close to magic bullets, they are not without gastrointestinal side effects like nausea, vomiting, diarrhea and constipation
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