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For amino acids with neutral sidechains, the pI can be calculated by averaging the pK a values of the carboxylic acid and ammonium groups For amino acids with acidic sidechains, the pI can be calculated by averaging the pK a values of the two most acidic groups For amino acids with basic sidechains, the pI can be calculated by averaging the pK a values of the least acidic groups Table of Contents Amino Acids as Zwitterions The Isoelectric Point, pI, Is the pH At Which Negative and Positive Charges are Balanced Calculating Isoelectric Point pI from pKa Values pI Calculations for Amino Acids With Acidic Side Chains pI Calculations for Amino Acids With Basic Side Chains Summary Notes Quiz Yourself
