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The next step of the cycle involves DHAR mediated reduction of DHA into AsA using GSH as the reductant (Figure 1)

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Cell lines were maintained at 37 C in a humidified incubator with 5% CO 2 and routinely tested for mycoplasma using MycoAlert Kit (Lonza)

Glutamine is converted to Glutamic Acid in the brain, which is essential for cerebral functions, and increases the amount of GABA (Gamma-aminobutyric acid), required for brain functioning and mental activity