GHK-Cu (Glycyl-L-Histidyl-L-Lysine Copper) is a copper-binding tripeptide first isolated from human plasma, studied in preclinical models for its effects on gene expression, extracellular matrix remodeling, and oxidative-stress biology
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The suspension was then solubilized by 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace) with 0.1% (w/v) cholesteryl hemisuccinate TRIS salt (CHS, Anatrace) for 3 h
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The percentage of recycled receptor was calculated by subtracting residual median fluorescence following the recycling protocol from that measured at t = 15 min, and normalizing to the percentage of internalized receptor at the same t = 15 min time-point