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Samples in extraction mix were vortexed for 10 min at 4 C and centrifuged at 15,000 rpm for 10 min at 4 C to pellet insoluble material

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Nikon A1R confocal microscope (Nikon, Japan) was applied to generate 3D rendered videos

10 To test whether the induction of p70S6K1 phosphorylation is due to inhibition of IGF-1R signaling pathway not non-specific outcome of OSI-906, the HCT116 cells were treated with other IGF-1R inhibitors, BMS-754807 and GSK1838705A, for 072 h

67 , 68 The GSH-antibiotic conjugate is more water-soluble and can be expelled, reducing the effective drug concentration