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Particle size, polydispersity coefficient (PDI), and zeta potential were measured using a nanoparticle size analyzer, and encapsulation efficiency was determined according to the method in Determination of liposome encapsulation rate and drug loading capacity

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Although the majority of readouts are at bulk level, mitochondrial changes in mass and morphology at single-cell resolution suggest an adaptive response rather than a selection for pre-existing tolerant cells in clonal lines