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Thus, the transition from crystal deposition to acute inflammation can be conceptualized as a two-hit process: First hit: MSU crystal deposition on cartilage surfaces activates synovial fibroblasts to release CCL2/MCP-1, recruiting monocytes and establishing subclinical inflammation

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B12 is also involved in the synthesis of hormones and neurotransmitters

MBio 13:e0325121

Response of gut microbiota and inflammatory status to bitter melon ( Momordica charantia L.) in high fat diet induced obese rats

The target Gram-positive organisms do not have an outer membrane