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Anaerobic samples were stored in glass screw top vials and N 2 -purged samples were transferred to pressure-relief scintillation vials kept under N 2 atmosphere via 27-gauge insulin syringe

Dong TA, Sandesara PB, Dhindsa DS, Mehta A, Arneson LC, Dollar AL, Taub PR, Sperling LS

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Allow the vial to reach room temperature, sterilise the stopper of both the compound vial and the bacteriostatic water vial with an alcohol swab, then draw the diluent into a sterile syringe and add it to the vial

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Duration of trials and organisms employed has varied, but there have been several instances of positive results (Rogler 2011)