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Transcript abundance also increased in infested resistant plants when the unique primer pair was used for qPCR analysis even though the variation was bigger than that when PCR was carried with the primer pair common to three different sequences among different replicates (Figure 2c)

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Nature 521 , 180185 (2015)

It's injecting air into the vial while drawing doses, which creates positive pressure that pulls contaminants back through the needle on every subsequent draw

Pyridoxamine, an inhibitor of advanced glycation and lipoxidation reactions: a novel therapy for treatment of diabetic complications

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