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The viral supernatant was harvested 48 h after the start of the transfection, filtered (0.45 m), and stored at 80C

To account for advancements in medical practice, we performed an additional analysis of patients enrolled before and after 2022

The following day medium was removed and replaced with 20 l of loading medium consisting of 1:1 (v/v) Opti-MEM: Hanks balanced salt solution (HBSS), 2.5% (v/v) FBS, 20 mM HEPES, pH 7.4, 2.5 mM probenicid and fluorescent indicator 2 M Fluo-4 AM (Invitrogen)

Summary: Injection sites for retatrutide include the abdomen, front of the thigh, and outer upper arm, administered subcutaneously in accordance with your clinical trial protocol, as retatrutide does not yet hold MHRA marketing authorisation