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Early on, the tissue is being actively re-signaled and needs the most consistent exposure, so activation is daily

He tinkered with various doses, often extrapolating from animal studies, and tried some on himself

Average normalised expression values for two biological replicates of RNAseq (12) are plotted for each life stage (egg, second-stage juvenile (J2) parasitic stages at 7, 14, 21, 28 and 35 days post infection (DPI) and non-parasitic adult males)

doi: 10.1016/S0140-6736(16)30173-8

Each treatment was performed in triplicate