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The frozen sections were rehydrated with phosphate-buffered saline (PBS) and then blocked with PBS containing 1% normal mouse serum, 1% bovine serum albumin, and 0.3% Triton X-100, followed by staining with directly conjugated antibodies for 24 h at room temperature in a dark, humidified chamber

Biol Pharm Bull (2018) 41(7):98593

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Histamine has been shown to stimulate, in a dose-dependent manner, the synthesis of estradiol (E2) (ref)