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doi: 10.1007/s00018-016-2194-1 18

Co-IP Procedure (using endogenous Co-IP as an example) Sample Preparation: Collect cells using an appropriate method and lyse them with Lysis Buffer containing suitable protease inhibitor cocktail to preserve protein-protein interactions

The expression levels of seleno-GPxs can be increased either through transcriptional induction or by elevating bioavailable selenium which supports their translation [8, 20, 24,25,26,27]

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Production of these metabolites must therefore be balanced against the availability of Cys and SAM

Bojkowski CJ, Arendt J, Shih MC, Markey SP