For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)
Standard oral glutathione has very low bioavailability a term that refers to how much of a substance actually enters the bloodstream to be used
Iron is a cofactor for fatty acid desaturases, essential for myelin synthesis
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This results in your body burning through its Glutathione - with the result that toxic by-products linger, and your hangover feels worse, for longer 27
Yale Journal Biology Medicine 89 (4), 513525