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Preparation of Tris-soybean lecithin extender The Tris-based extender was formulated by dissolving 3.025 g Tris, 1.66 g citric acid monohydrate, and 1.25 g glucose in distilled water, followed by the addition of 1% (v/v) soybean lecithin, 7% (v/v) glycerol, 100 g/mL lincomycin, and 100 g/mL streptomycin