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The diluent should be introduced slowly into the vial, directed down the inner wall to minimise mechanical disruption to the peptide

[1] Cell Line:Cultured Sprague-Dawley rat Achilles tendon fibroblasts (passages 2-4) Concentration:0, 0.5, 1, 2 g/mL Incubation Time:24 h Result:Did not alter total FAK, paxillin, or PCNA protein levels

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This date is determined by stability studies that show how long the solution (including the benzyl alcohol) remains stable and effective