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2 Methods 2.1 Search strategy The present systematic review adhered to the guidelines outlined in the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) (Moher, 2009)

Analysis of glutathione content was performed using a one-way ANOVA, and significance between groups was determined using Tukey's post hoc tests

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Delivery of therapeutics Viral delivery of CRISPR-Cas9 gene constructs and ASOs have been a commonly used method that utilizes vectors such as lentiviruses, adenoviruses, and adeno-associated viruses (AAV) [141] (Table 1)

It deserves emphasis that to date there is no rigorous evidence for a programmed neurotoxic astrocyte phenotype that is somehow activated in a common manner across multiple disorders and represents a universal target, in spite of certain claims to this effect