Cell culture All cell lines (K7M2 (CRL-2836), 143B (CRL-8303), HOS (CRL-1543)) were obtained from the American Type Culture Collection (ATCC)
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Ru(II)-OH with a concentration of 50 M was incubated with different concentrations of GSH substrates (250 M, 500 M, 1000 M and 2000 M)
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Purified DNA was denatured by heating and MeDIP and hMeDIP were conducted with 5mC or 5hmC antibodies as we previously described 64